9PI9

Sacituzumab Fab bound to Trop2 Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977410
Spacegroup name	P 1 21 1
Unit cell lengths	50.430, 210.600, 78.060
Unit cell angles	90.00, 105.56, 90.00

Refinement procedure

Resolution	47.340 - 1.560
R-factor	0.1811
Rwork	0.181
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	1.600
High resolution limit [Å]	1.559	1.560
Rmerge	0.044	0.976
Rmeas	0.061	1.345
Rpim	0.042	0.921
Number of reflections	426530	29462
<I/σ(I)>	8.39	0.76
Completeness [%]	99.6	97.7
Redundancy	1.8	1.7
CC(1/2)	0.998	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	100 mM NaCitrate, 18% PEG3350