9OU2

K-Ras Wild Type 1-169 Bound to BI-2865 at 100 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 2 21
Unit cell lengths	40.474, 55.440, 87.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.750 - 1.550
Rwork	0.224
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.403
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.750	1.570
High resolution limit [Å]	1.550	1.550
Rpim	0.017
Number of reflections	29414	1295
<I/σ(I)>	77.5	4
Completeness [%]	97.3
Redundancy	1.9
CC(1/2)	0.997	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	25% PEG2000, 0.2 mM magnesium chloride, 100 mM sodium acetate, pH 4.6