9OIO

The von Hippel Lindau-ElonginB-ElonginC (VCB) complex with fragments 9 and 14

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 41 2 2
Unit cell lengths	92.904, 92.904, 359.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.490 - 2.300
R-factor	0.2132
Rwork	0.211
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	2.330
High resolution limit [Å]	2.300	2.300
Rmerge	0.119	2.022
Rpim	0.024	0.387
Number of reflections	71258	2699
<I/σ(I)>	19.12	2.03
Completeness [%]	99.9	99.96
Redundancy	26.5	28
CC(1/2)	1.000	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium cacodylate pH 5.5, 0.2 M magnesium acetate, 5 mM DTT and 14-20% PEG8000