9OC6

Crystal structure of receptor FcRn bound to Human Astrovirus 6 spike

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	211.925, 76.682, 53.807
Unit cell angles	90.00, 98.55, 90.00

Refinement procedure

Resolution	29.140 - 2.970
R-factor	0.2331
Rwork	0.228
R-free	0.28050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	FAST_DP
Data scaling software	FAST_DP
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.140	3.050
High resolution limit [Å]	2.970	2.970
Rmerge	0.152	0.821
Number of reflections	17653	1254
<I/σ(I)>	10.7
Completeness [%]	99.6
Redundancy	7.1
CC(1/2)	0.995	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES, 30% 1,2-propanediol, 20% PEG-400