9O3L

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, deacylated A-site tRNAphe, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAphe at 2.75A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	208.760, 447.070, 617.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.560 - 2.750
R-factor	0.2306
Rwork	0.228
R-free	0.28180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvk
RMSD bond length	0.004
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.560	122.560	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.222	0.045	2.091
Rmeas	0.240	0.049	2.262
Total number of observations	10167837
Number of reflections	1472876	17246	108492
<I/σ(I)>	7.83	29.64	0.94
Completeness [%]	99.8	98.7	99.8
Redundancy	6.903	6.914	6.861
CC(1/2)	0.995	0.998	0.130

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME