9O03
Crystal structure of AfOgg1:8OG-DNA duplex in a product-bound state
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2001-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.813, 44.815, 59.026 |
| Unit cell angles | 90.00, 104.29, 90.00 |
Refinement procedure
| Resolution | 35.200 - 2.000 |
| R-factor | 0.2203 |
| Rwork | 0.219 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.525 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.094 | |
| Number of reflections | 19200 | 1402 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.996 | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20-30% PEG4000, 100 mM MES, pH 6.5, 5% potassium permanganate (saturated) |
