9NN9

ISG15 complexed with nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	P 1 21 1
Unit cell lengths	68.460, 114.470, 79.290
Unit cell angles	90.00, 105.86, 90.00

Refinement procedure

Resolution	76.270 - 2.590
R-factor	0.1915
Rwork	0.188
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.039
Data reduction software	XDS (VERSION Jun 30, 2024 BUILT=20241002)
Data scaling software	XSCALE (VERSION Jun 30, 2024 BUILT=20241002)
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.700
High resolution limit [Å]	2.590	2.600
Rmerge	0.134	0.826
Rmeas	0.159	0.983
Rpim	0.085	0.527
Number of reflections	36396	2168
<I/σ(I)>	6.91	1.26
Completeness [%]	99.3	95.69
Redundancy	3.4
CC(1/2)	0.992	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	25 % PEG 3350, 0.1 M trisodium citrate pH 5.6, 0.2 M ammonium sulfate