Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2024-07-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.880, 58.080, 64.870 |
| Unit cell angles | 111.21, 99.57, 109.92 |
Refinement procedure
| Resolution | 33.100 - 2.050 |
| R-factor | 0.2083 |
| Rwork | 0.206 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.639 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.100 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 35892 | 3524 |
| <I/σ(I)> | 9.24 | |
| Completeness [%] | 98.9 | 97.67 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 0.857 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291.15 | 0.2 M Sodium malonate pH 4.0, 20% w/v Polyethylene glycol 3,350 |
