9N8E
Crystal structure of EBOV glycoprotein with modified HR1c (L579P) and HR2 stalk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 114.474, 114.474, 133.381 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.570 - 3.200 |
| R-factor | 0.2157 |
| Rwork | 0.215 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.570 | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 17104 | 815 |
| <I/σ(I)> | 10.67 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.998 | 0.212 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M sodium citrate-citric acid pH4.5 ,12% PEG-6000 |
