9N50
Crosslinked Crystal Structure of Human Mitochondrial Ketosynthase, OXSM, and Crosslinker-crypto Human Mitochondrial Acyl Carrier Protein, C8aBr-mACP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.947, 119.437, 78.874 |
| Unit cell angles | 90.00, 103.29, 90.00 |
Refinement procedure
| Resolution | 62.980 - 2.500 |
| Rwork | 0.220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.060 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 41771 | 4691 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.995 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M ammonium sulfate, 1 M lithium sulfate, and 0.1 M sodium citrate pH 5.6 |
