9N4C

RhoA GTPase E102A bound to GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-12-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	54.217, 60.532, 67.134
Unit cell angles	67.84, 78.05, 68.36

Refinement procedure

Resolution	44.260 - 2.300
R-factor	0.1794
Rwork	0.177
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.974
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.260	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.093	0.246
Rmeas	0.131	0.348
Rpim	0.019	0.093
Number of reflections	53737	3053
<I/σ(I)>	27.53	8.85
Completeness [%]	96.3	68.39
Redundancy	3.9	3.8
CC(1/2)	0.984	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2 uL protein (8 mg/mL RhoA E102A bound to GTPgammaS, 25 mM Tris-HCl pH 8.0, 2 mM MgCl2, 10 mM BME) + 2 uL of crystallization solution over 500 uL reservoir of crystallization solution. Crystallization solution: 0.2 M Ammonium iodide, 20% PEG 3,350