9N20

Structure of C3d Bound to a Fragment of FHR-2 and S. aureus Efb-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-21
Detector	MAR CCD 300 mm
Wavelength(s)	1.00
Spacegroup name	P 61
Unit cell lengths	145.584, 145.584, 76.855
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.740 - 3.300
R-factor	0.1818
Rwork	0.178
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.457
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.214	0.054	1.556
Rmeas	0.220	0.055	1.631
Rpim	0.048	0.012	0.472
Number of reflections	14125	1468	1392
<I/σ(I)>	4.3
Completeness [%]	100.0	100	99.6
Redundancy	20.8	22.2	10.7
CC(1/2)	1.000	0.998	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M imidazole [pH 6.7] 12% (w/v) peg-20k