9N1F

Crystal Structure of the Ark2C-Ubc13~Ub-Mms2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-23
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.953700
Spacegroup name	C 2 2 21
Unit cell lengths	73.735, 100.415, 142.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.000 - 2.100
Rwork	0.229
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.091	1.459
Rmeas	0.094	1.510
Rpim	0.025	0.389
Number of reflections	31139	3060
<I/σ(I)>	18.16	2.12
Completeness [%]	99.9	100
Redundancy	14.5	14.9
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Sodium Malonate and 18% w/v PEG 3350