9MX8
Crystal structure of the DNA binding domain of FLI1 in complex with a DNA containing three contiguous GGAA sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.539, 91.539, 180.728 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.670 - 3.150 |
| R-factor | 0.25133 |
| Rwork | 0.250 |
| R-free | 0.27833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jvt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.078 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 10335 | 775 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10 | |
| CC(1/2) | 1.000 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M sodium cacodylate, pH 7.0, 1.6 M sodium acetate, added cryoprotectant: 34% sucrose |
