9MWY
Crystal structure of the DNA binding domain of FLI1 in complex with a DNA containing four contiguous GGAA sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.201, 78.201, 318.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.280 |
| R-factor | 0.21721 |
| Rwork | 0.214 |
| R-free | 0.28126 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jvt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.330 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Number of reflections | 16164 | 753 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 10.1 | |
| CC(1/2) | 1.000 | 0.989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M Tris, pH 6.5, 0.7 M sodium acetate |
