9MTS

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-unmodified Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.033210
Spacegroup name	P 21 21 21
Unit cell lengths	210.490, 452.410, 626.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	366.790 - 2.700
R-factor	0.2206
Rwork	0.218
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvl
RMSD bond length	0.004
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	366.790	366.790	2.770
High resolution limit [Å]	2.700	12.090	2.700
Rmerge	0.244	0.049	1.622
Rmeas	0.271	0.056	1.807
Total number of observations	8443502
Number of reflections	1601309	18789	111943
<I/σ(I)>	6.49	22.37	1.02
Completeness [%]	99.5	98.7	94.4
Redundancy	5.273	5.098	5.065
CC(1/2)	0.981	0.996	0.178

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME