9MTP

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	208.400, 447.210, 624.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.080 - 2.400
R-factor	0.2247
Rwork	0.223
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvl
RMSD bond length	0.004
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	107.080	107.080	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.175	0.026	2.078
Rmeas	0.191	0.028	2.266
Total number of observations	13185074
Number of reflections	2111059	24060	158999
<I/σ(I)>	10.13	45.98	0.9
Completeness [%]	94.4	91.4	96.4
Redundancy	6.246	6.584	6.169
CC(1/2)	0.995	0.999	0.161

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME