9MJY
Crystal structure of WRN helicase with bound allosteric compound 1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2022-11-19 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.666, 57.857, 130.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.790 - 1.630 |
| R-factor | 0.18839 |
| Rwork | 0.188 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.171 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.790 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 54424 | 2761 |
| <I/σ(I)> | 16.9 | 1.4 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 8.1 | 8.3 |
| CC(1/2) | 0.999 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 21%-27.5% (w/v) PEG 1500 and 0.1 M MMT pH 5.75-6.25 |
