9MJX
Crystal structure of WRN helicase with bound allosteric fragment 4
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2022-11-19 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.440, 58.061, 130.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.450 - 1.730 |
| R-factor | 0.1995 |
| Rwork | 0.199 |
| R-free | 0.22933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.414 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 46408 | 2276 |
| <I/σ(I)> | 13.1 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 21%-27.5% (w/v) PEG 1500 and 0.1 M MMT pH 5.75-6.25 |
