9MFG

Complex of IL23 receptor and VHH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	103
Detector technology	PIXEL
Collection date	2022-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	105.313, 403.472, 84.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.970 - 2.850
R-factor	0.206
Rwork	0.204
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.751
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.900
High resolution limit [Å]	2.850	7.710	2.850
Rmerge	0.123	0.057	0.507
Rmeas	0.146	0.067	0.659
Rpim	0.077	0.034	0.415
Total number of observations	225294
Number of reflections	74785	4392	1970
<I/σ(I)>	6
Completeness [%]	87.8	95.3	47.1
Redundancy	3	3.6	1.7
CC(1/2)	0.988	0.993	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	0.1 M Tris (pH 8.5), 16% 606 PEG4000, 200 mM Li2SO4, 200 mM NaSCN and was cryoprotected with 25% glycerol