9MEZ

Crystal structure of MRAS(GDP) bound to LZTR1(Kelch domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97627
Spacegroup name	P 1 21 1
Unit cell lengths	70.764, 110.788, 223.253
Unit cell angles	90.00, 94.53, 90.00

Refinement procedure

Resolution	49.220 - 2.800
R-factor	0.2164
Rwork	0.214
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.606
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.097	1.554
Rpim	0.061	0.958
Number of reflections	84708	4499
<I/σ(I)>	9.7	1
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.997	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	11.2% w/v PEG3350, 5.26% Tacsimate pH 6.0