9MEY

Crystal structure of RIT1(GDP) bound to LZTR1(Kelch domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 31 2 1
Unit cell lengths	148.582, 148.582, 130.728
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.070 - 2.950
R-factor	0.2193
Rwork	0.217
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.070	3.090
High resolution limit [Å]	2.950	2.950
Rmerge	0.075	1.471
Rmeas	0.083
Rpim	0.035	0.681
Number of reflections	35502	4675
<I/σ(I)>	18.4	1.8
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	1.000	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	277	44% Morpheus II PPT7, 100mM MonoSach II, 100mM MB6 buffer pH 8.2