9M6G

the crystal structure of the Ca2+/CaM-CASK-CaMK-Mint1-CID complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	61.214, 80.562, 109.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.740 - 1.700
R-factor	0.1665
Rwork	0.165
R-free	0.18710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9m5y
RMSD bond length	0.015
RMSD bond angle	1.341
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge		0.048	0.614
Rmeas		0.051	0.645
Rpim		0.016	0.195
Number of reflections	60114	6300	5935
<I/σ(I)>	12.4
Completeness [%]	99.9	99.4	100
Redundancy	10.8	10.4	10.7
CC(1/2)	0.998	0.998	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	0.1 M Sodium malonate, pH 5.0, 12% (v/v) PEG 3350