9M4N
Structure of AtCDC48
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980118 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 144.467, 144.467, 162.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.290 - 3.450 |
| R-factor | 0.3482 |
| Rwork | 0.346 |
| R-free | 0.38380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 3.730 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.620 | 3.770 |
| Rmeas | 0.640 | 3.880 |
| Rpim | 0.150 | 0.920 |
| Number of reflections | 13805 | 13799 |
| <I/σ(I)> | 8.4 | 1.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 31.8 | 32.5 |
| CC(1/2) | 0.990 | 0.320 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM HEPES, 20mM magnesium chloride hexahydrate, 22% poly acrylic acid 5100, pH 7.5 |
