9LW4
UbCh8-ISG15 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-28 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.87 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.984, 32.313, 107.042 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 65.025 - 2.700 |
| Rwork | 0.232 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.475 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431 (refmacat 0.4.126)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.820 | 9.000 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 18182 | 2196 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 97.7 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.994 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES pH 6.5 and 15% w/v PEG 20000 |
