9LUY
Single-chain Fv antibody of G2 fused with antigen peptide from chicken chromosome 6 C10orf76 homolog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.054, 71.390, 43.352 |
| Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
| Resolution | 31.140 - 1.620 |
| R-factor | 0.1679 |
| Rwork | 0.167 |
| R-free | 0.18810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pgx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.051 | 0.712 |
| Number of reflections | 28848 | 1429 |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.656 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES monohydrate pH 6.5, 12% w/v polyethylene glycol 20,000 |
