9LTH

Crystal structure of transcriptional regulator (NrpR) from Streptococcus salivarius K12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9797
Spacegroup name	I 1 2 1
Unit cell lengths	34.747, 128.260, 72.630
Unit cell angles	90.00, 100.62, 90.00

Refinement procedure

Resolution	36.705 - 2.590
Rwork	0.183
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.454
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.705	2.710
High resolution limit [Å]	2.590	2.590
Number of reflections	9474	2958
<I/σ(I)>	11.8
Completeness [%]	97.4
Redundancy	2.5
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.1 M Bis-Tris propane (pH 7.95), 0.7 M Sodium Citrate tribasic dihydrate