9LQH

Crystal structure of SAM lyase in complex with sinefungin-AMP

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	153
Detector technology	PIXEL
Collection date	2024-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	47.160, 53.880, 153.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.540 - 1.470
R-factor	0.1896
Rwork	0.188
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.045
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.120	1.510
High resolution limit [Å]	1.470	1.470
Number of reflections	66545	4681
<I/σ(I)>	16.6
Completeness [%]	98.8
Redundancy	10.6
CC(1/2)	0.997	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	0.1 M Sodium acetate trihydrate pH 4.0, 15% v/v Polyethylene glycol 400