9LOI

Crystal Structure of the Heterodimeric HIF-2 Complex with Agonist SD-16

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-16
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	49.642, 99.609, 77.660
Unit cell angles	90.00, 107.08, 90.00

Refinement procedure

Resolution	37.120 - 2.780
R-factor	0.2229
Rwork	0.220
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.240	2.930
High resolution limit [Å]	2.780	2.780
Rmerge	0.118	1.800
Rmeas	0.129
Rpim	0.052	0.782
Number of reflections	17079	2676
<I/σ(I)>	9
Completeness [%]	93.3
Redundancy	6.1	6.4
CC(1/2)	0.997	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 3350, Succinic Acid pH 7.0