Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-06 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.348, 79.685, 162.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.467 - 2.200 |
| R-factor | 0.227479941172 |
| Rwork | 0.224 |
| R-free | 0.29345 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wqa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.949 |
| Data reduction software | CrysalisPro (171.39.46) |
| Refinement software | REFMAC (V8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.470 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.149 | 0.368 |
| Number of reflections | 37773 | 3820 |
| <I/σ(I)> | 13.9 | 3 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M HEPES (pH 7.4), 0.1 M MgCl2, 12-15% PEG3350, 10% isopropanol, and 25% ethylene glycol |
