9LMZ
hAGO2-MID in complex with a chemical modified uridine monophosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.561, 46.536, 66.277 |
| Unit cell angles | 86.95, 73.95, 85.04 |
Refinement procedure
| Resolution | 46.340 - 1.800 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.340 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | |
| Rmeas | 0.049 | |
| Rpim | 0.034 | |
| Number of reflections | 40082 | 5883 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 93.0 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.998 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.25M Ammonium sulfate 0.1M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8,000 |
