9LFF
Crystal structure of UgpB in complex with G1P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.200, 114.910, 46.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.340 - 1.500 |
| R-factor | 0.1815 |
| Rwork | 0.181 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.340 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.090 | 1.210 |
| Number of reflections | 63229 | 4198 |
| <I/σ(I)> | 18.7 | 1.7 |
| Completeness [%] | 99.0 | 90 |
| Redundancy | 12.4 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M PCB buffer (pH 5.0) and 25% (w/v) PEG 1500 |
