9LDE
The complex structure of K63A/2'-p-ADPR-RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.253, 89.708, 66.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.790 - 1.970 |
| R-factor | 0.1806 |
| Rwork | 0.178 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.949 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.190 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.104 | 0.521 |
| Rmeas | 0.113 | 0.706 |
| Rpim | 0.043 | 0.472 |
| Total number of observations | 148224 | 2302 |
| Number of reflections | 25041 | 1563 |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 82.1 | |
| Redundancy | 5.9 | 1.5 |
| CC(1/2) | 0.990 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 300 | 40 mM Citric acid 60 mM BIS-TRIS propane pH 6.4 20% w/v PEG 3350 |
