9L8A

Structure of frog TREX2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-01-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	60.678, 62.355, 105.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.180 - 2.050
R-factor	0.2221
Rwork	0.220
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tqn
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.180	2.100
High resolution limit [Å]	2.050	2.050
Rpim	0.031	0.222
Number of reflections	25220	2044
<I/σ(I)>	27.2	4
Completeness [%]	97.0	80.3
Redundancy	12.8	12.5
CC(1/2)	0.992	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M MES pH 6.5, 5% PEG400