9L7E
Crystal structure of human kinesin-1 motor domain (G234A mutant) in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.360, 67.930, 55.450 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 19.550 - 2.400 |
| R-factor | 0.196 |
| Rwork | 0.192 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg2 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.052 | 0.412 |
| Number of reflections | 14330 | 1056 |
| <I/σ(I)> | 17.9 | 3.73 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 23% PEG 3350, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
