9KVO
Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation D132E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.888, 83.365, 124.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.090 - 3.140 |
| R-factor | 0.28804 |
| Rwork | 0.282 |
| R-free | 0.34498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.090 | 3.180 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmeas | 0.257 | |
| Rpim | 0.119 | 0.385 |
| Number of reflections | 8061 | 657 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.977 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1M Tris (pH8.5), 0.2M Sodium acetate trihydrate, 32% PEG4000 |
