9KUU

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation D130G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	39.607, 84.318, 125.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.380 - 2.500
R-factor	0.2002
Rwork	0.193
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.893
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.380	2.592
High resolution limit [Å]	2.500	2.502
Rmeas	0.189	1.000
Rpim	0.078	0.488
Number of reflections	14537	1342
<I/σ(I)>	8.6
Completeness [%]	96.1
Redundancy	5.6
CC(1/2)	0.993	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1 M Tris (pH 8.5), 0.2 M Sodium acetate trihydrate, 30% (w/v) PEG4000