9KRJ

Structure of mouse Dyrk1a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-11-03
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.540, 88.120, 148.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.690 - 3.000
R-factor	0.186
Rwork	0.183
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.272
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.690	3.107
High resolution limit [Å]	3.000	3.000
Rpim	0.164
Number of reflections	18335	1789
<I/σ(I)>	17.54
Completeness [%]	99.9
Redundancy	14.3
CC(1/2)	0.849	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2M Lithum Sulfate, 0.1M Tris:HCl (pH 8.5), 40% (v/v) PEG 400