9KOT
HEWL crystal soaked in buffer of pH2.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-17 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.034, 79.034, 36.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.930 - 1.500 |
| R-factor | 0.18135 |
| Rwork | 0.179 |
| R-free | 0.22661 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.720 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.930 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | |
| Number of reflections | 17815 | 1240 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 97.4 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | The concentration of the protein was 25 mg/ml. The precipitant solution in the reservoir was 100 mM sodium citrate/HCl buffer of pH 4.3 containing 1.2 M sodium chloride. |
