Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9735 |
| Spacegroup name | H 3 |
| Unit cell lengths | 140.316, 140.316, 83.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.560 - 2.900 |
| R-factor | 0.2208 |
| Rwork | 0.219 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.480 |
| Data reduction software | HKL-3000 |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmeas | 0.430 | 0.244 |
| Number of reflections | 13366 | 11923 |
| <I/σ(I)> | 32.7 | |
| Completeness [%] | 87.6 | |
| Redundancy | 2.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM sodium citrate(pH5.8), 15% PEG3350, 8% isopropanol. |
