9KH4

Crystal structure of Galectin-8 N-CRD with N-acetyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 43 21 2
Unit cell lengths	49.154, 49.154, 160.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.530 - 1.750
R-factor	0.1816
Rwork	0.178
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.785
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.530	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.078	0.392
Number of reflections	20585	1138
<I/σ(I)>	27.4
Completeness [%]	98.6
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	PEG