9K6J

Crystal structure of SARS-CoV-2 WT RBD bound with P5-1C8 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	55.306, 115.092, 144.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.440 - 2.390
R-factor	0.2381
Rwork	0.236
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdi
RMSD bond length	0.004
RMSD bond angle	0.777
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.940	7.550
High resolution limit [Å]	2.390	2.390
Rmerge	0.086
Rmeas	0.091
Number of reflections	34150	34150
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	9
CC(1/2)		0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	0.2 M Ammonium phosphate dibasic,28% w/v Polyethylene glycol 3,350