9K0J

Crystal structure of FGFR4 kinase domain with 43b

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2022-04-21
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 1 21 1
Unit cell lengths	42.280, 61.543, 59.987
Unit cell angles	90.00, 97.73, 90.00

Refinement procedure

Resolution	42.755 - 2.068
R-factor	0.1679
Rwork	0.166
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.734
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.760	2.141
High resolution limit [Å]	2.068	2.068
Rmerge	0.189	0.329
Rmeas	0.203
Number of reflections	18740	1832
<I/σ(I)>	9.46	4.79
Completeness [%]	99.8	98.07
Redundancy	7.2	5.7
CC(1/2)	0.987	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, and 19% PEG3350