9JXW
Crystal structure of SiRe_0806 from Sulfolobus islandicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.929, 95.623, 108.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.770 - 3.000 |
| R-factor | 0.266 |
| Rwork | 0.264 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.770 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.079 | 0.309 |
| Number of reflections | 18726 | 2991 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M sodium acetate trihydrate, pH=4.76; 2.0 M ammonium sulfate |
