9JUS

Structure of villin bound to an actin trimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	98.137, 102.432, 147.128
Unit cell angles	77.95, 72.59, 65.96

Refinement procedure

Resolution	29.900 - 2.700
Rwork	0.196
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ffk 1h1v
RMSD bond length	0.002
RMSD bond angle	0.499
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.920	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.081	0.123
Rmeas	0.096
Rpim	0.051	0.756
Number of reflections	133624	13218
<I/σ(I)>	7.2	1
Completeness [%]	98.3	97.9
Redundancy	3.5	3.6
CC(1/2)		0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.1 M Bis-Tris propane, pH 6.5 10% PEG 3350 0.2 M KSCN 5 mM CaCl2/MgCl2