9JQU

The crystal structure of SFTSV Gn and SD4 antibody complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	96.856, 46.360, 97.322
Unit cell angles	90.00, 113.30, 90.00

Refinement procedure

Resolution	32.210 - 3.300
R-factor	0.252
Rwork	0.250
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y10 7l2e
RMSD bond length	0.004
RMSD bond angle	0.789
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Number of reflections	11858	11716
<I/σ(I)>	5.675
Completeness [%]	97.4
Redundancy	4.5
CC(1/2)	0.971	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate pH 4.6, 30% w/v polyethylene glycol 4,000