9JH2

Crystal Structure of NFIA in Complex with DNA containing the TGGCA Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2024-03-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	94.388, 41.021, 70.901
Unit cell angles	90.00, 95.41, 90.00

Refinement procedure

Resolution	40.930 - 2.300
R-factor	0.2432
Rwork	0.241
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9jh4
RMSD bond length	0.003
RMSD bond angle	0.506
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.048	0.356
Rmeas	0.052	0.390
Rpim	0.021	0.155
Number of reflections	12244	1767
<I/σ(I)>	41.9
Completeness [%]	99.2	99.9
Redundancy	6.37	6.12
CC(1/2)	0.994	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris Propane (pH 7.0), and 1.5 M Ammonium Sulfate