9JEJ

Crystal structure of human EP300 KIX domain (L644C mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	I 4 2 2
Unit cell lengths	96.342, 96.342, 136.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.260 - 2.900
R-factor	0.2542
Rwork	0.251
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	7414	339
<I/σ(I)>	20.286
Completeness [%]	99.8	99.7
Redundancy	7.6
CC(1/2)	0.995	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium Chloride, 0.1 M Sodium Citrate : Citric Acid pH5.5, 1 M Ammonium Phosphate Dibasic