9JCX

Crystal structure of the HCoV-HKU1 RBD and TMPRSS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	63.350, 120.720, 126.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.750
R-factor	0.207
Rwork	0.205
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.950	19.950	2.910
High resolution limit [Å]	2.750	7.680	2.750
Number of reflections	25815	1227	4018
<I/σ(I)>	15.24
Completeness [%]	99.6
Redundancy	13.27
CC(1/2)	0.998	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.2 M Tris pH 7.0, 0.2 M MgCl2, 10% (w/v) Polyethylene glycol 8,000