9J7I
Cryo-EM Structure of calcium sensing receptor in complex gamma-glutamyl-valyl-glycine as a kokumi substance
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 3.4 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.55 |
| Software | REFMAC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
